BioTek Synergy 2

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The Synergy 2 is a multi-mode plate reader. It is capable of reading Fluorescence Intensity, Fluorescence Polarization, Time-Resolved Fluorescence, AlphaScreen, Luminescence and UV-visible Absorbance.

The absorbance detection system is monochromator-based, providing the best level of flexibility in this read mode. The instrument has dedicated optical elements for each individual detection technique.

Our Synergy 2 is equipped with a Dual Reagent Dispensers for readings coordinated with the addition of chemical stimulus (instantaneous or specifically timed). This is an important feature for luciferase assays.

General Information:

Sample Input:

  • Sample preparations in 96 or 384 well plates
  • Our Synergy 2 is equipped with Dual Reagent Dispensers

  • We have these filters and mirrors:

  • Filters:


    • 340/30 (NADH, DAPI, Hoechst, LanthaScreen europium, and Propidium iodide excitation)
    • 440/40 (Attophos, Pacific Green, and Lucifer Yellow excitation)
    • 485/20 (AlexaFluor488, FITC, GFP, and PicoGreen excitation)
    • 530/25 (CY3 and 5-Tamra excitation)
    • 560/15 (Alamar Blue, Cell Titer Blue, mCherry, RFP, and Texas Red excitation)
    • 620/40 (AlexaFluor633 and CY5 excitation)


    • 440/40 (Caspace-3, DAPI, and Hoechst emission)
    • 485/20 (CFP and Pacific Green emission)
    • 528/20 (AlexaFluor488, FITC, GFP, PicoGreen, and Lucifer Yellow emission)
    • 590/20 (CY3, Cell Titer Blue, Alamar Blue, and RFP emission)
    • 570/100 (AlphaScreen emission)
    • 635/32 (mCherry, Propidium iodide and Texas Red emission)
    • 680/30 (CY5 emission)


    • Top 50%
    • Dichroic Top 570 nm
    • Dichroic Top 595 nm
    • Dichroic Top 635 nm
  • If you need different filters for your assay please contact us.

  • Expected Results:
  • Fluorescence Intensity, Fluorescence Polarization, Time-Resolved Fluorescense, AlphaScreen, Luminescence, or UV-visible Absorbance data.

  • More Information


Plate Selection Tips:

  • For luminescence

    • A solid bottom white plate is recommended because it will maximize your signal

  • For fluorescence

    • Black plates reduce background noise

    • A solid bottom black plate is recommended if you will be doing a top read (most assays)

    • A clear bottom black plate is recommended if you will be doing a bottom read of adherent cells

    For absorbance

    • A clear bottom black plate is required


UofA Internal: $21  |  Academic / Nonprofit: $25.20 | Industry: $36.75  |  Unit: Per Hour

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