Attune Acoustic Focusing Cytometer


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The Attune Acoustic Focusing Flow Cytometer has both high sensitivity and high throughput. The autosampler can sample from 96 well or 384 well microplates. You can control your sample concentration, the flow rate, and the number of photons you detect.

The acoustic focusing technology uses ultrasonic waves (over 2 MHz, similar to those used in medical imaging), rather than hydrodynamic forces, to position cells into a single, tightly focused line along the central axis of a capillary at the point of laser interrogation regardless of the sample-to-sheath ratio. Watch the cool video below.

Watch the 5 minute Attune Tutorial

Instrument Specifications:

Excitation lasers:

Violet 405 nm (50 mW)    


Blue 488 nm (20 mW)

Emission Filters:    

Violet 1 450/40 (430-470)  


Blue 1 530/30 (515-545)

Violet 2 522/31 (507-537)

Blue 2 574/26 (561-588)

Violet 3 603/48 (579-627)

Blue 3 640LP  (>640)

Tools to design you assay:

LifeTech Fluorescence SpectraViewer

BD Biosciences Spectrum Viewer

Reagent Selection Guide for the Attune Cytometer.

Download the Attune Software and analyse your data on your own computer.


Looking for a different Cytometer?  Try The Cytometry Core Facility

General Information:

  • Sample Input:

    Live or fixed cells in individual tubes or 96 well or 384 well microplates, or run some samples in tubes and others in plates 

  • Expected Results:

    High quality flow cytometry data

    Download the Attune's free software that allows you to analyze data from any computer, anywhere, at any time. 

    See the Attune Software Demo


  • Sample Preparation Details:

    The Attune is a very sensitive and flexable flow cytometry platform. It can accomodate live or fixed cells in individual tubes or 96 well or 384 well microplates, or some samples in tubes and others in plates.

  • Price:

    UofA Internal: $38  |  Academic / Nonprofit: $45.60 |  Industry: $66.50  |  Unit: Per Hour

  • Related Services:

    BioTek Synergy 2

    Operetta CLS