The CLARIOstar Plus is both the most flexible and sensitive microplate reader on the market. This machine is capable of reading fluorescence, luminescence, and absorbance in 6-1536 well plates. The CLARIOstar Plus has patented Linear Variable Filters that allow the user to select their ideal wavelengths with the provided and supported software, allowing for excitation and emission reads over multiple wavelengths without hardware manipulations. The CLARIOstar Plus is able to perform modern cell/molecular/high throughput assays including:
- DNA,RNA and Protein quantification
- Cell based assay / Long Term Kinetic Live Cell Assays
- Enzyme and Kinetic assays
- Genotyping & Reporter gene assays
- Molecular binding assays
- ELSIA
- Fluorescence Polarization
- Bioluminescent Resonance Energy Transfer (BRET)
- Time Resolved Fluorescence (TRF)
Sample Input:
- Sample preparations in 6-384 well plates
- The CLARIOstar Plus is equipped with Dual Reagent Dispensers (Dead volume 600ml)
Light Sources:
- High energy xenon flash lamp
- Dedicated Laser for Alphascreen / AlphaLISA / Alphaplex
Detection Modes:
- UV/Vis absorbance
- Fluorescence intensity
- Luminescence
- Fluorescence polarization
- Time-resolved fluorescence
- TR-FRET
- Alphascreen / AlphaLISA / Alphaplex
Measurement Modes:
- Top and bottom reading
- Endpoint and Kinetic
- Sequential multi-excitation
- Sequential multi-emission
- Spectral scanning (Absorbance, Luminescence, Fluorescence)
- Ratiometric measurements
- Well scanning
Spectral Range:
- LVF Monochromators:
- Fluorescence: 320-740 nm Luminescence: 320-740 nm
- Additional Filters:
-
- Ex TR
- LP TR
- LP 504
- LP 566
- LP 601
- LP 639
- SP AS
- 482-16
- 530-40
- 540-20
- 570-100
- 580-10
- 590-20
- 590-50
- 615-18
- 620-10
- 665-10
- 680-40
- 875-50
- Linear Variable Dichroic
- 340-740nm
- Spectrometer:
- ABS:220-1000nm
Plate Selection Recommendations:
- For luminescence
- A solid bottom white plate is recommended because it will maximize your signal
- For fluorescence
- Black plates reduce background noise
- A solid bottom black plate is recommended if you will be doing a top read (most assays)
- A clear bottom black plate is recommended if you will be doing a bottom read of adherent cells
For absorbance
-
- A clear bottom plate is required