Operetta CLS

 

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The Operetta CLS high-content analysis system has all the speed, sensitivity and resolution you need to characterize fine sub-cellular details . The Operetta is designed for fully-automated high-capacity, quantitative cellular analysis of fixed or live cells. It contains the highly sensitive sCMOS camera that provides a large field of view and high resolution image capture. The Operetta's Harmony software lets you find even subtle phenotypic changes.

Features and Benefits:

  • Objectives
    • 5x Air Objective: NA 0.16, WD 12.1 mm (field of view: approx. 2583 μm x 2583 μm)
    • 10x Air Objective: NA 0.3, WD 5.2 mm (field of view: approx. 1292 μm x1292 μm)
    • 20x Air Objective: NA 0.4, WD 8.28 mm (field of view: approx. 646 μm x 646 μm)
    • 20x Water Immersion Objective: NA 1.0, WD 1.7 mm (field of view: approx. 646 μm x 646 μm)
    • 40x Water Immersion Objective: NA 1.1, WD 0.62 mm (field of view: approx. 323 μm x 323 μm)
    • 63x Water Immersion Objective: NA 1.15, WD 0.6 mm (field of view: approx. 205 μm x 205 μm)
  • 4.7Mpx sCMOS camera (2160x2160), 16 bit resolution, 6.5um pixel size
  • Motorized 6 position objective turret with 3 air and 3 water immersion objectives
  • High resolution, linear drive scanning stage
  • High speed precision laser autofocus
  • Transmitted light unit (LED: 740nm) for Brightfield and Digital Phase Contrast imaging
  • Emission filters:
    • 430-500 nm
    • 470-515 nm
    • 500-550 nm
    • 525-580 nm
    • 570-650 nm
    • 655-760 nm
  • Spinning Disk enables optical sectioning
  • Confocal imaging improves optical resolution and contrast
  • Pinhole disk reduces out-of-focus light
  • The LED light engine is equipped with 8 high power LEDs with the following excitation wavelengths:
    • 355-385 nm
    • 390-420 nm
    • 435-460 nm
    • 460-490 nm
    • 490-515 nm
    • 530-560 nm
    • 615-645 nm
    • 650-675 nm
  • Environmental Controls: Sample chamber fitted with temperature and CO2 control to enable live cell experiments.
    • Temperature can be controlled between 37°C - 42°C
    • CO2 concentration can be controlled between 1-10%

Tools to design you assay:

LifeTech Fluorescence SpectraViewer

BD Biosciences Spectrum Viewer

Price:

  • UofA Internal: $55 |  Academic / Nonprofit: $75.26 |  Industry: $110.25  |  Unit: Per Hour